Coding
MerA

Part:BBa_K4831001

Designed by: Joakim Gustafsson   Group: iGEM23_ABOA-Turku   (2023-10-01)


MerA (mercuric reductase) from Pseudomonas aeruginosa (seq. harmonized to Synechocystis)

Overview

MerA protein catalyzes the reduction of mercuric ion (Hg2+) to elemental mercury. The merA -gene codes for mercuric reductase (EC 1.16.1.1) that catalyzes the reduction of toxic ionic mercury (Hg2+) to less volatile and toxic elemental mercury (Hg0). It can be used as a combination with merB, which is a organomercury lyase. Together or alone, merA can be used to increase the resistance to organic mercury (methyl mercury) or mercuric compounds like mercuric (II)chloride. It can be used as a tool for bioremediation.

Design Notes

The original sequence is harmonized for optimal translation in Synechocystis sp PCC 6803. (changed codons: 406, GC-change: 65.8 to 56.0%).

NOTE! The starting codon ATG has been removed to be compatible with our composite part (reading frame shift).

Source

Part is from Pseudomonas aeruginosa genomic sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1198
    Illegal NgoMIV site found at 1246
    Illegal AgeI site found at 1484
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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